TNFR1&TNFR2 Dual Effector Reporter Cell
CBP74618
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| I. Background | |
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TNFR1和TNFR2是肿瘤坏死因子-α（TNF-α）的两种受体，共同介导TNF的多种生物学功能。TNFR1（p55） 在几乎所有细胞中广泛表达，顺利获得胞内的死亡结构域主要介导炎症反应和细胞凋亡。TNFR2（p75） 表达相对局限，主要在免疫细胞上，顺利获得激活NF-κB和AKT通路介导细胞存活、增殖和免疫调节功能。 |
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| II. Description | |
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TNFR1&TNFR2 Dual Effector Reporter Cell 药靶模型很好的模拟了体内 TNFR1&TNFR2 的信号转导过程。 |
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| III. Introduction | |
| Expressed gene: | TNFR1&TNFR2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+0.05 mM 2-Mercaptoethanol |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 1. Recombinant TNFR1&TNFR2 Dual Effector Reporter Cell constitutively expressing TNFR1&TNFR2.  Figure 2. Blocking of TNFα induced TNFR1&TNFR2 Dual Effector Reporter Cell Activity by T'NFα Neutralization Ab or TNFR1/R2 Blocking Abs with Membrane Anchored TNFα Cell (Adherent, C12).  Figure 3. Inhibition of Human Recombinant TNFα Induced Reporter Activity by TNFα Neutralization Ab or TNFR1/R2 Blocking Abs in TNFR1&TNFR2 Dual Effector Reporter Cell. |
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